![]() ![]() Can use wright’s stain or special stain for eosinophils like Methylene-blue and eosin-Y in methanol.On wet preparation, eosinophils are difficult to recognize except for the presence of 2 or 3 lobes.It may be seen few along with neutrophils. Eosinophils usually are not seen in the urine sediment.WBCs clumps are a sign of infection and must be reported.>30 cells /HPF is considered an infection.RBCs are seen as biconcave, non-nucleated discs measuring 7 µm in diameter.The presence of RBC in the urine is called Hematuria.Variable in males and females but more in females 1 to 5 /HPF The following table shows the normal contents of the sediments:.Normally there is no sediment or very little.The standard centrifugation speed recommended is 400 g.The recommended time for centrifugation is 5 minutes.Centrifuge the Urine, clear supernatant is removed and leaves the uniform volume of sediment, usually 1 mL.Urine culture gives false-positive results when contaminated with stool, vaginal secretions, and contaminated test tubes or hands and clothes.False proteinuria is seen when urine is contaminated with vaginal secretions.Vaginal secretions contamination may cause false-positive leucocyte esterase.Traumatic catheterization may cause hematuria and false RBCs in the urine.Females may have contamination from the vaginal secretions and show an increased number of WBCs.When radiopaque contrast media is given to the patient will give false-positive urine protein for at least 3 days.A high protein, a fat diet without the intake of carbohydrates also causes ketonuria.A high protein diet and some foods (cranberries) make urine acidic.Eating citrus fruits, dairy products, and vegetables makes urine alkaline.If you keep urine on the table, bacteria will change the urine pH, make it alkaline because of urea splitting bacteria and there is the production of the ammonia.Asparagus produces a typical urine odor.Rhubarb will change its color to red or brown.Carrots will change their urine color to dark yellow.There are certain foods that will color the urine like:.The best volume for the centrifuge is 10 to 12 mL.įactors that will interfere with the urine analysis:.If delayed, then refrigerate the urine.Freshly voided urine is the best sample.Urine analysis Urine Microscopic Examination Sample for urine analysis
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